Isolation and partial characterization of two different heat-stable enterotoxins produced by bovine and porcine strains of enterotoxigenic Escherichia coli

R A Kapitany, G W Forsyth, A Scoot, S F McKenzie, R W Worthington
1979 Infection and Immunity  
Heat-stable enterotoxins (ST-124 and ST-1261) have been isolated from two different enterotoxigenic Escherichia coli of bovine (124) and porcine (1261) origin. The enterotoxin preparations were isolated by ultrafiltration and ionexchange chromatography and were both active in the suckling mouse test and pig ligated loop test in the nanogram range. The bovine (ST-124) enterotoxin was not stable to heating in its isolated form, and significant differences in amino acid composition were observed
more » ... tween the two enterotoxins. Although both toxins were active at similar levels in the suckling mouse and pig ligated loop tests, ST-124 lacked the ability to cause the profound secretary responses seen with ST-1261 in the weanling pig ligated loop.
doi:10.1128/iai.26.1.173-177.1979 fatcat:ufzklvieu5ev7jlqin6pfi4j7i