In this study, we investigated the relationship between catalase activity and H2O2induced hormetic response in budding yeast S. cerevisiae. In general, our data suggest that: (i) hydrogen peroxide induces hormesis in a concentration-and time-dependent manner; and (ii) the effect of hydrogen peroxide on yeast colony growth positively correlates with the activity of catalase that suggests the enzyme involvement in overall H2O2-induced stress response and hormetic response in yeast.