Organogenesis in zygotic embryos and hypocotyls of physic nut (Jatropha curcas L.) under 6-benzylaminopurine levels

Jefferson Da Luz Costa, André Luís, Lopes Da Silva, Sidnei Emilio, Bordignon Júnior, Aloísio Freitas, Chagas Júnior, Gessiel Scheidt, Ronaldo Valadares, Veras Júnior
2014 Adv. For. Sci   unpublished
Physic nut had a great potential for biodiesel production and presents higher oil content. The aim of this study was obtain organogenesis in zygotic embryos and hypocotyls of physic nut using 6-benzylaminopurine (BAP). Seeds were disinfected and after then cotyledons were removed; they were in vitro germinated at 0, 1, 2, 3, 4, 5 and 6 mg.L-1 BAP. Hypocotyls were excised from germinated seeds with 45 days old and cultured at 0, 1, 2.5 and 5 mg.L-1 BAP. Callus from zygotic embryos were cultured
more » ... ryos were cultured at double-phase medium being liquid medium supplemented with 1, 2.5 and 5 mg.L-1 BAP. The largest percentage of callus formation in zygotic embryos occurred at 6 mg.L-1 BAP. On the other hand, to induce shoot from zygotic embryos the best BAP level was 4 mg.L-1. Callogenesis in hypocotyls can be efficiently induced with 1 mg.L-1 BAP. Double-phase medium results in lowest protuberances rate, varying from 0 to 3 per callus. In order to induce callus from zygotic embryos and hypocotyls of physic nut, BAP is sustainable.