Global analysis of mRNA stability in Mycobacterium tuberculosis

Tige R. Rustad, Kyle J. Minch, William Brabant, Jessica K. Winkler, David J. Reiss, Nitin S. Baliga, David R. Sherman
2012 Nucleic Acids Research  
Mycobacterium tuberculosis (MTB) is a highly successful pathogen that infects over a billion people. As with most organisms, MTB adapts to stress by modifying its transcriptional profile. Remodeling of the transcriptome requires both altering the transcription rate and clearing away the existing mRNA through degradation, a process that can be directly regulated in response to stress. To understand better how MTB adapts to the harsh environs of the human host, we performed a global survey of the
more » ... decay rates of MTB mRNA transcripts. Decay rates were measured for 2139 of the $4000 MTB genes, which displayed an average half-life of 9.5 min. This is nearly twice the average mRNA half-life of other prokaryotic organisms where these measurements have been made. The transcriptome was further stabilized in response to lowered temperature and hypoxic stress. The generally stable transcriptome described here, and the additional stabilization in response to physiologically relevant stresses, has far-ranging implications for how this pathogen is able to adapt in its human host.
doi:10.1093/nar/gks1019 pmid:23125364 pmcid:PMC3592478 fatcat:r36hho23tbg77khwf4fituei2y