Thermal Activation of the Antiviral Activity of Synthetic Polyribonucleotides: Influence of DEAE-dextran in Various Cell Cultures

E. de Clercq, T. C. Merigan
1971 Journal of General Virology  
After heating at 37 ° in minimal Eagle's medium, the alternating polyriboadenylicpolyribouridylic acid [poly r(A-U)] became much more active in producing cellular resistance to virus infection and interferon production in four different cell lines (human skin fibroblasts, mouse embryo fibroblasts, mouse L 929 cells and rabbit kidney (RK 13) cells). The thermal activating effect was neutralized by pre-treatment of the cells with DEAE-dextran, which increased the antiviral activity of unheated
more » ... vity of unheated and decreased the activity of heated poly r(A-U) to the same level. Under all conditions tested, the degree of cellular resistance to virus infection closely paralleled the amounts of interferon produced suggesting that interferon production is responsible for the antiviral resistance produced by poly r(A-U).
doi:10.1099/0022-1317-10-2-125 pmid:4324254 fatcat:h2rasxlgjjhsvpm3meh4edmq5y