Nitric Oxide Accelerates Mycorrhizal Effects on Plant Growth and Root Development of Trifoliate Orange

Li Tian, Nasrullah Nasrullah, Xiao-Yun Huang, Qiang-Sheng Wu
2017 Sains Malaysiana  
Arbuscular mycorrhizal fungi (AMF) actively colonize plant roots and thus enhance plant growth through different mechanisms. In the present study, trifoliate orange (Poncirus trifoliata) seedlings inoculated with Diversispora versiformis were subjected to 0 and 0.2 mmol/L sodium nitroprusside (SNP, a nitric oxide donor) treatments. After eight weeks, exogenous SNP considerably increased root mycorrhizal colonization by 25%, showing a positive stimulating effect of NO on mycorrhizal formation.
more » ... corrhizal inoculation significantly increased plant growth performance (height, stem diameter, leaf number and shoot and root dry weight) and root traits (length, projected area, surface area, volume and number of 2 nd and 3 rd order lateral roots) than non-mycorrhizal treatment and NO (exogenous SNP treatment) heavily strengthened the mycorrhizal effects. Moreover, NO and mycorrhization induced more fine root (0-0.5 cm) formation. There was an opposite changed trend in root sucrose and leaf and root glucose contents by SNP in AMF versus non-AMF seedlings. All these results implied that NO plays important roles in mycorrhizal formation and development and also accelerates mycorrhizal effects on plant growth and root development of trifoliate orange. ABSTRAK Kulat mikoriza arbuskula (AMF) mengkoloni akar tumbuhan secara aktif dan seterusnya menggalakkan pertumbuhan pokok melalui mekanisme berbeza. Dalam kajian ini, benih oren trifoliat (Poncirus trifoliata) yang diinokulasi dengan Diversispora versiformis telah diberikan rawatan 0 dan 0.2 mmol/L sodium nitropussida (SNP, penderma nitrik oksida). Selepas lapan minggu, SNP eksogenus didapati meningkatkan pengkolonian akar mikoriza sebanyak 25% dan ini menunjukkan kesan rangsangan positif NO terhadap pembentukan mikoriza. Penginokulasian mikoriza meningkatkan prestasi pertumbuhan pokok secara signifikan (tinggi, diameter batang, jumlah daun dan berat akar kering) dan ciri akar (panjang, luas unjuran, luas permukaan, isi padu, bilangan akar lateral peringkat ke-2 dan ke-3) berbanding rawatan tanpa mikoriza serta NO (rawatan SNP eksogenus) mengukuhkan lagi kesan mikoriza. Di samping itu, rawatan NO dan mikoriza mengaruh lebih banyak pembentukan akar halus (0-0.5 cm). Terdapat trend perubahan bertentangan pada kandungan sukrosa akar, daun serta glukosa akar oleh SNP dalam benih AMF berbanding tanpa AMF. Keseluruhan keputusan kajian ini menunjukkan bahawa NO memainkan peranan penting dalam pembentukan dan perkembangan mikoriza, malah mempercepatkan kesan mikoriza ke atas pertumbuhan pokok dan perkembangan akar oren trifoliat.
doi:10.17576/jsm-2017-4610-03 fatcat:mnm3htrutveong7gpoyqi7lzlm