Memory destabilization and reconsolidation dynamically regulate the PKMζ maintenance mechanism [article]

Matteo Bernabo, Karim Nader
2020 bioRxiv   pre-print
Useful memory must balance between stability and malleability. This puts effective memory storage at odds with plasticity processes like reconsolidation. What becomes of memory maintenance processes during synaptic plasticity is unknown. Here we examined the fate of the memory maintenance protein PKMζ during memory destabilization and reconsolidation. We found that NMDA receptor activation and proteasome activity induced a transient reduction in PKMζ protein following retrieval. During
more » ... al. During reconsolidation, new PKMζ was synthesized to re-store the memory. Failure to synthesize new PKMζ during reconsolidation impaired memory but uninterrupted PKMζ translation was not necessary for maintenance itself. Finally, NMDA receptor activation was necessary to render memories vulnerable to the amnesic effect of PKMζ-antisense. These findings outline a transient collapse and renewal of the PKMζ memory maintenance mechanism during plasticity. We argue that dynamic changes in PKMζ protein levels can serve as an exemplary model of the molecular changes underlying memory destabilization and reconsolidation.
doi:10.1101/2020.07.04.187823 fatcat:lyn7vfuvubcqpozxitvc4wbc6y