Rapamycin Enhances HBV Production by Inducing Cellular Autophagy

Wenjuan Huang, Fengrong Zhao, Ying Huang, Xia Li, Sufei Zhu, Qin Hu, Weixian Chen, Fengrong Zhao, Ying Huang, Xia Li
2014 Hepatitis Monthly  
Some reports revealed that rapamycin could reactivate HBV infection. However, the mechanism has not been clearly explained. In this report, we studied the mechanism by which rapamycin enhances HBV replication and expression by inducing cellular autophagy. HepG2.2.15 cells were treated with rapamycin to induce autophagy. Autophagosomes were observed by fluorescence microscopy and transmission electron microscopy. Autophagy marker protein LC3-Ⅱ/LC3-Ⅰwas detected by Western blotting. HBV DNA and
more » ... NA were determined by real time PCR and Southern blotting. HBsAg was evaluated by ELISA. In HepG2.2.15 cells, HBV DNA and HBsAg increased when host cells were treated with rapamycin and the effect was reversed by autophagy inhibitor, 3-methyladenine (3-MA). These results indicated a potential explanation for reactivation of HBV infection when patients with hepatitis receive rapamycin.
doi:10.5812/hepatmon.20719 pmid:25419217 pmcid:PMC4238155 fatcat:4jy5sjfyebb35b5vqqnajifrrq