Genome-wide transposon mutagenesis of paramyxoviruses reveals constraints on genomic plasticity

Satoshi Ikegame, Shannon M. Beaty, Christian Stevens, Sohui T. Won, Arnold Park, David Sachs, Patrick Hong, Benhur Lee, Patricia A. Thibault, Anice C. Lowen
2020 PLoS Pathogens  
The antigenic and genomic stability of paramyxoviruses remains a mystery. Here, we evaluate the genetic plasticity of Sendai virus (SeV) and mumps virus (MuV), sialic acid-using paramyxoviruses that infect mammals from two Paramyxoviridae subfamilies (Orthoparamyxovirinae and Rubulavirinae). We performed saturating whole-genome transposon insertional mutagenesis, and identified important commonalities: disordered regions in the N and P genes near the 3' genomic end were more tolerant to
more » ... nal disruptions; but the envelope glycoproteins were not, highlighting structural constraints that contribute to the restricted antigenic drift in paramyxoviruses. Nonetheless, when we applied our strategy to a fusion-defective Newcastle disease virus (Avulavirinae subfamily), we could select for F-revertants and other insertants in the 5' end of the genome. Our genome-wide interrogation of representative paramyxovirus genomes from all three Paramyxoviridae subfamilies provides a family-wide context in which to explore specific variations within and among paramyxovirus genera and species.
doi:10.1371/journal.ppat.1008877 pmid:33035269 fatcat:ljgqkbyjn5hkpj3wr3bb7p76ie