Tumor-infiltrating CD4+ T lymphocytes in early breast cancer reflect lymph node involvement

Alexandre Henrique Macchetti, Heitor Ricardo Cosiski Marana, João Santana Silva, Jurandyr Moreira de Andrade, Alfredo Ribeiro-Silva, Sérgio Bighetti
2006 Clinics  
S. Tumor-infiltrating CD4 + T lymphocytes in early breast cancer reflect lymph node involvement. Clinics. 2006;61(3):203-8. BACKGROUND: The role of immune system in the pathogenesis and progression of breast cancer is a subject of controversy, and this stimulated us to investigate the association of the immunophenotype of tumor-infiltrating lymphocytes in early breast cancer with the spread of tumor cells to axillary lymph nodes. METHODS: Tumor samples from 23 patients with early breast cancer
more » ... arly breast cancer from the Department of Gynecology and Obstetrics of Ribeirão Preto Medical School (USP) were obtained at the time of biopsy and submitted to an enzyme-digestion procedure for the extraction of tumor-infiltrating lymphocytes. The lymphocytes extracted were analyzed by dual-color flow cytometry with monoclonal antibodies in these combinations: CD3 FITC/CD19 PE, CD3 FITC/CD4 PE, CD3 FITC/CD8 PE, and CD16/56 PerCP, which are specific for immunophenotyping of T and B lymphocytes, helper and cytotoxic T lymphocytes, and natural killer (NK) cells. The mean percentage of these cells was used for comparing groups of patients with or without lymph node metastasis. RESULTS: The mean value for T-lymphocyte infiltration was 24.72 ± 17.37%; for B-lymphocyte infiltration, 4.22 ± 6.27%; for NK-cell infiltration, 4.41 ± 5.22%, and for CD4 + and CD8 + T-lymphocyte infiltration, 12.43 ± 10.12% and 11.30 ± 15.09%, respectively. Only mean values of T-and CD4 + T-lymphocyte infiltration were higher in the group of patients with lymph node metastasis, while no differences were noted in the other lymphocyte subpopulations. CONCLUSION: The association of tumor-infiltrating CD4 + T lymphocytes with lymph node metastasis suggests a role for these cells in the spread of neoplasia to lymph nodes in patients with early breast cancer. CLINICS 2006;61(3):203-8 Tumor-infiltrating CD4 + T lymphocytes in early breast cancer reflect lymph node involvement Macchetti AH et al.
doi:10.1590/s1807-59322006000300004 fatcat:podrcbhgsbc3bbfgjd53me3vbi