HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2

Vania Maslarska, Meri Amerikova, Ivanka Pencheva, Stanislav Bozhanov
2020 Indian Journal of Pharmaceutical Education and Research  
Aim: The aim of this research study was to develop a simple, rapid, precise, accurate and economical RP-HPLC method, with a simple mobile phase for the identification and determination of the antimicrobial peptide α-defensin-2. Materials and Methods: Separation was carried out at 25°C, using column Luna 5U (C 18 , 250x4.6, 5 μm) with mobile phase consisting of acetonitrile: water (40: 60 v/v). The detector was set at 210 nm. The flow rate was 1.2 ml/min and injection volume was 20 μl. Results:
more » ... he developed method was validated as per ICH guidelines and the kinetic behavior of α-defensin 2 was studied in respect of different pH. Conclusion: The obtained data can serve for creating an in vivo monitoring program and quality control for different studies and preparations.
doi:10.5530/ijper.54.2.53 fatcat:5d6zbcoqnngubgzcwhmfjnq7iq