Schisandrin B Induces Apoptosis and Cell Cycle Arrest of Gallbladder Cancer Cells

Shan-Shan Xiang, Xu-An Wang, Huai-Feng Li, Yi-Jun Shu, Run-Fa Bao, Fei Zhang, Yang Cao, Yuan-Yuan Ye, Hao Weng, Wen-Guang Wu, Jia-Sheng Mu, Xiang-Song Wu (+7 others)
2014 Molecules  
Gallbladder cancer, with high aggressivity and extremely poor prognosis, is the most common malignancy of the bile duct. The main objective of the paper was to investigate the effects of schisandrin B (Sch B) on gallbladder cancer cells and identify the mechanisms underlying its potential anticancer effects. We showed that Sch B inhibited the viability and proliferation of human gallbladder cancer cells in a dose-, time -dependent manner through MTT and colony formation assays, and decrease
more » ... s, and decrease mitochondrial membrane potential (ΔΨm) at a dose-dependent manner through flow cytometry. Flow cytometry assays also revealed G0/G1 phase arrest and apoptosis in GBC-SD and NOZ cells. Western blot analysis of Sch B-treated cells revealed the upregulation of Bax, cleaved caspase-9, cleaved caspase-3, cleaved PARP and downregulation of Bcl-2, NF-κB, cyclin D1 and CDK-4. Moreover, this drug also inhibited the tumor growth in nude mice carrying OPEN ACCESS Molecules 2014, 19 13236 subcutaneous NOZ tumor xenografts. These data demonstrated that Sch B induced apoptosis in gallbladder cancer cells by regulating apoptosis-related protein expression, and suggests that Sch B may be a promising drug for the treatment of gallbladder cancer.
doi:10.3390/molecules190913235 pmid:25165862 fatcat:my544sym5fbrhapbv67nm6qt74