Novel IL-6-secreting γδT cells increased in patients with atherosclerotic cerebral infarction

YOUDI HE, NING MA, CHEN XING, XIAOQIAN WANG, HE XIAO, MINGKE ZHENG, GENCHENG HAN, GUOJIANG CHEN, CHUNMEI HOU, BEIFEN SHEN, YAN LI, ZHENYU JIANG (+2 others)
2014 Molecular Medicine Reports  
Mounting evidence has suggested that inflammation associated with interleukin (IL)-6 and T-helper (Th)17 cells, has a role in the development of atherosclerotic cerebral infarction (ACI). However, it remains unclear which population of cells determines the levels of IL-6, and the role of IL-6-secreting cells in inducing Th17 cell production. In the present study, IL-6 levels were determined in patients with ACI, by ELISA. The percentage of CD3 + T, CD4 + T, CD8 + T, CD11c + dendritic cells and
more » ... endritic cells and γδT cells were determined by flow cytometry, and the correlation between cytokine IL-6 and γδT cells was determined by statistical analysis. An in vitro culture assay was used to determine whether γδT cells secreted high levels of IL-6, and induced production of Th17 cells. The patients with ACI had significantly higher levels of IL-6 and γδT cells. Furthermore, γδT cells were associated with the secretion of a high level of IL-6 in patients with ACI. These results indicate that γδT cells are novel IL-6-secreting cells, which from then on were known as γδT6 cells. In addition, the novel γδT6 cells induced Th17-cell production, and this induction was dependent on IL-6. Novel γδT6 cells increased the induction of Th17-cell production in patients with ACI. The results of the present study suggest that novel γδT6 cells may be a target for strategic therapies of ACI.
doi:10.3892/mmr.2014.2796 pmid:25351283 fatcat:hyattql5rvbpbckqnr4tr3rjdy