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Introduction In gas chromatographic-mass spectroscopic analysis of buprenorphine metabolites, the urine specimen must be first hydrolyzed to release buprenorphine and norbuprenorphine from their glucuronide conjugates. For evaluation of existing hydrolysis methods and to find out the optimal hydrolysis conditions, buprenorphine-3-1~-D-glucuronide (B3G) and norbuprenorphine-3-II-D-glucuronide (NB3G) were synthesized. In a previous communication we reported on the optimum conditions fordoi:10.1093/jat/29.6.570 pmid:16168181 fatcat:u6j43uxxdba55h7yz7veyybvzu