P190Deficiency of non-endothelial eNOS causes hypertension in mice

T Suvorava, J Stegbauer, M Thieme, S Pick, C Rump, JW Fischer, T Hohlfeld, G Kojda
2014 Cardiovascular Research  
Purpose: Despite irrefutable role of endothelial nitric oxide synthase (eNOS) in vascular smooth muscle tone, its contribution to the regulation of blood pressure has been proven mainly either by the global eNOS-deficiency or by the use of unspecific NOS-inhibitors. To distinguish the relevance of endothelial and non-endothelial eNOS, we generated a novel double transgenic mouse model lacking eNOS in all organs except the vascular endothelium. The aim of the study was to evaluate the
more » ... uate the contribution of non-endothelial eNOS to the regulation of blood pressure (BP). Methods: To accomplish this a double transgenic strain expressing eNOS exclusively in the vascular endothelium has been generated (eNOS-Tg/KO) by endothelial-specific Tie-2 driven targeting of bovine eNOS in eNOS-deficient mice (eNOS-KO). Results: Expression of eNOS was evaluated in aorta, myocardium, kidney, brain stem and skeletal muscle ofeNOS-Tg/KO bywesternblot and real-time PCR. Organbathstudiesrevealed a completenormalization of aortic reactivity to acetylcholine (ACh), phenylephrine and the NO-donors SNAP and DEA/NO in
doi:10.1093/cvr/cvu082.125 fatcat:roctpi6mszegbmdjaavjn3lvpa