Vitrification of Mice Embryos in Different Developmental Stages Using Four Vitrification Methods

Nicolae Păcală, Alexandra Ivan, Ada Cean
2012 Biotechnology & Biotechnological Equipment  
The aim of our study was to test four vitrification methods for preservation of mice embryos, in different developmental stages, using 6 mol/l glycerol and 20 % sucrose as vitrification media. Morula stage embryos were vitrified with OPS straws or classic straws using two equilibration steps, with a hatching rate of 50 % and 47.62 % respectively. Blastocyst stage embryos survived better in 0.25 ml straws with three equilibration steps, 36.84 % hatching rate. Two-cell embryos are sensitive to
more » ... are sensitive to freezing, but satisfactory results could be obtained with OPS vitrification, hatching rates of 22.73 %. The success of the vitrification technique depends on the developmental stage of the embryo and the method used.
doi:10.5504/bbeq.2012.0078 fatcat:egrhn6tczna3vgdsf27upqxrhi