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The aim of our study was to test four vitrification methods for preservation of mice embryos, in different developmental stages, using 6 mol/l glycerol and 20 % sucrose as vitrification media. Morula stage embryos were vitrified with OPS straws or classic straws using two equilibration steps, with a hatching rate of 50 % and 47.62 % respectively. Blastocyst stage embryos survived better in 0.25 ml straws with three equilibration steps, 36.84 % hatching rate. Two-cell embryos are sensitive todoi:10.5504/bbeq.2012.0078 fatcat:egrhn6tczna3vgdsf27upqxrhi