Deoxypodophyllotoxin (DPT), or anthricin, is a lignan isolated from the roots of Anthriscus sylvestris and is reported to exhibit anti-inflammatory, anti-oxidant, and anti-asthmatic effects. Herein, the conditions for the extraction of DPT from A. sylvestris are optimized using a Box-Behnken design (BBD) method based on response surface methodology (RSM). DPT was detected by ultra-performance liquid chromatography coupled with photodiode array and quadrupole detector (UPLC-PDA-QDa) and

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... l validation methods based on International Conference on Harmonization (ICH) guidelines. In preliminary experiments, the experimental conditions of extraction time, solvent percentage, and temperature were selected for optimization. The adequacy of the experimental model was statistically evaluated, and the regression coefficient (R 2 ), adjusted regression coefficient (R 2 adjust ), and p-value of the lack-of-fit were determined as 97.86%, 94.02%, and 0.124, respectively. The maximum yield of DPT was estimated to be 2.341 mg/g for 30 min in 100% methanol at 60 °C, and the actual yield was measured as 2.295 mg/g (±0.023) under the same conditions.