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Validation of Analytical Method for Quantification of Egg Cholesterol Using Reversed Phase-High Performance Liquid Chromatography-Multiwavelength Detector
Tropical Animal Science Journal
In this research, analytical method of cholesterol content in eggs by Reversed Phase-High Performance Liquid Chromatography-Multiwavelength Detector (RP-HPLC-MWD) was validated. Our experiment validated the modified method of AOAC 994.10:2012 to get a more simple and efficient analytical method of cholesterol content. The sample was saponified using 10% KOH concentration for 15 min at 80 °C, then this analytical method was validated. RP-HPLC-MWD condition was at 100% MeOH as a mobile phase,doi:10.5398/tasj.2019.42.3.230 fatcat:wp7mdhgj5bebforyddin5kjt7y