The seeds of Gleditsia sinensis is one of the most effective drugs for the treatment of esophageal carcinoma. To definite the active ingredients of the seeds of gleditsia sinensis, SEGS was extracted from the seeds of Gleditsia sinensis. Investigations the interaction between the active ingredient of traditional Chinese medicine and BSA is helpful for explaining the medicine mechanism. Under the simulative human physiological conditions, the interaction between SEGS and bovine serum albumin

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... ) was investigated by fluorescence spectroscopy. The quenching mechanism of SEGS with BSA is dynamic quenching, SEGS bound with BSA by van der Waals forces or hydrogen bond. The binding constants and the number of binding sites between SEGS and BSA at different temperatures (298 and 308 K) were obtained.