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DNA Sequencing with [α-33P]-Labeled ddNTP Terminators: A New Approach to DNA Sequencing with ThermoTM Sequenase DNA Polymerase
A new approach to DNA sequencing is described. The method is based on the use of [ α -33 P]-labeled dideoxyribonucleoside triphosphate terminators and Thermo Sequenase ™DNA polymerase in cycle sequencing. Thermo Sequenase DNA polymerase incorporates ddNTPs as efficiently as dNTPs, allowing the use of low concentrations of these nucleotides in DNA sequencing. Because only the properly terminated chains are labeled and visualized on autoradiography of the sequencing gels, the sequence results aredoi:10.2144/96216pf01 pmid:8969845 fatcat:trnzqflswvdkpi5ikdtcfrhlgu