New genetic resources for mammalian developmental biologists

David Beier
2010 F1000 Biology Reports  
The utilization of homologous recombination in embryonic stem cells as a means to generate mice carrying pre-determined modifications of genomic sequences has revolutionized the study of developmental biology. Recognizing the potential efficiencies that can be obtained by high-throughput production at centralized technology centers, a number of large-scale efforts for generating mice with targeted mutations have been funded. These programs are reaching fruition, and a variety of libraries of
more » ... ryonic stem cells with defined mutations are now available.
doi:10.3410/b2-72 pmid:21173845 pmcid:PMC2989628 fatcat:7vxs2vadtrgvrojtoorkjuy4re