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Assessment of mapping strategies for determining the 5ʹ-end of mRNAs and long-noncoding RNAs with short read sequences
[article]
2020
bioRxiv
pre-print
Genome mapping is an essential step in data processing for transcriptome analysis, and many previous studies have evaluated various methods and strategies for mapping RNA-seq data. Cap Analysis of Gene Expression (CAGE) is a sequencing-based protocol particularly designed to capture the 5'-ends of transcripts for quantitatively measuring the expression levels of transcription start sites genome-wide. Because CAGE analysis can also predict the activities of promoters and enhancers, this protocol
doi:10.1101/2020.03.14.982991
fatcat:id7dk2weh5fm7lymqautpbvcha