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Recebido em 23/9/11; aceito em 27/11/11; publicado na web em 28/2/12 Prior to obtain a standardized dried extract from V. ferruginea, lupeol was first time isolated from leaves and used as chemical maker. An analytical method using HPLC-PDA for lupeol determination in V. ferruginea intermediate products was developed using a C8 reverse-phase column, acetonitrile-acetic acid (99.99:0.01, v/v) as mobile phase at 0.8 mL min -1 , oven temperature at 23-25 °C, sample injection volume at 30 μL anddoi:10.1590/s0100-40422012000500032 fatcat:7rxq4du7vzbc3jmaodmnan4mmu