Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob

Ezequiane Machado Silva Oliveira, Sabrine Lemes Freitas, Frederico Severino Martins, Renê Oliveira Couto, Mariana Viana Pinto, José Realino Paula, Edemilson Cardoso Conceição, Maria Teresa Freitas Bara
2012 Química Nova  
Recebido em 23/9/11; aceito em 27/11/11; publicado na web em 28/2/12 Prior to obtain a standardized dried extract from V. ferruginea, lupeol was first time isolated from leaves and used as chemical maker. An analytical method using HPLC-PDA for lupeol determination in V. ferruginea intermediate products was developed using a C8 reverse-phase column, acetonitrile-acetic acid (99.99:0.01, v/v) as mobile phase at 0.8 mL min -1 , oven temperature at 23-25 °C, sample injection volume at 30 μL and
more » ... ection at 210 nm. The method presented linearity from 10 to 160 μg mL -1 , accuracy, precision, robustness and suitable sensitivity proving to be a useful tool to the obtainment process of lupeol standardized dried extracts of V. ferruginea.
doi:10.1590/s0100-40422012000500032 fatcat:7rxq4du7vzbc3jmaodmnan4mmu