Vitamin D Status Among Women Living in Sulaimani, Kurdistan Region and HPLC-MS Analysis for MeasuringVitamin D in Organic and Non-organic Eggs
Kirkuk University Journal-Scientific Studies
Vitamin D is a suparstar of our health. Sun exposure is the major source of vitamin D for most humans, and the primary cause of vitamin D deficiency in women living in Kurdistan is the lack of appreciation of the sun. Very few foods naturally known to contain vitamin D, and these foods are not a part of these women daily meals. In the first section of this study, the measured serum 25(OH), D levels for 1128 women and girls were considered. Over 79% of these results were below the standard range
... the standard range value. In the second part of this study, we use analysis with the Hight Performance Liquid Chromatography Mass Spectrometry (HPLC-MS) method to elucidate the ratio of Vitamin D between commercially produced eggs (non-organic eggs) and Vitamin D in eggs produced by hens raised on pasture (organic eggs). Data showed vitamin D content in organic eggs 7.66 fold higher than non-organic eggs. It is clear that attention should pay to the vitamin D status of women in Kurdistan. Two ml acetonitrile was added to the precipitate and solicited for 2 minutes until dissolved. The mixture was centrifuged at 14000 rpm for 5 minutes and then filtered by passing through a Millipore micro filter with 0.2 micrometer pores. Separation was carried out with an HPLC-MS (Waters 2545-USA) Quaternary Gradient Module, and it equipped with system fluidics organizer (Waters-SFO).It coupled with SQ detector and operated in positive ionization mode at range from m/z = 200-900 with two scans/min, combined with Photodiodes Array detector (Waters 2998) with sampling rate 2 points/sec (Lambda range 190-800 nm). A C18/4.5 x 155 mm RP column (XBridge) was used for separation with a solvent system consisting of A: Deionized water, B: Acetonitrile each contains 0.1% formic acid. The following gradient was applied: 0-2 minutes 30% A, and 70% B, 2-10 minutes a linear from 25 to 5%, B linear from 75% to 95%. Ten microliters of the extract were injected using auto-sampler (Waters 2767), and run with flow rate 1 ml/min using auto-sampler (Waters 2767). Vitamin D was identified in the samples by comparison to the retention time and MS data and retention time of official standard. RESULTS AND DISCUSSION The usual range of Serum concentrations of 25-hydroxy Vitamin D is between (30 and50) ng/ml, Level between (10-20) ng/ml is deficient, and range between (5 and10) ng/ml is sever deficiency.