Rapid impedance measurement of tethered bilayer lipid membrane biosensors

Xiaoyi Mu, D. Rairigh, Xiaowen Liu, A. J. Mason
2011 2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society  
Tethered bilayer lipid membranes (tBLM) offer a promising means to immobilize membrane proteins for sensor applications and study biological phenomena including membrane-nanoparticle interactions. tBLM biointerfaces are typically characterized using electrochemical impedance spectroscopy (EIS) in the 1mHz to 1Hz range due to interface parasitics. To enable rapid characterization of biointerfaces for high throughput applications, this paper introduces a method for high resolution EIS
more » ... ion EIS characterization of tBLMs at higher frequencies. The tBLM equivalent electrical model is analyzed, and the benefit of extracting the real portion of interface admittance is described. Mathematical analysis shows that the maximum frequency for measuring membrane resistance is a function of membrane characteristics and that small area membranes could enable measurement well into the kHz range, permitting observation of millisecond membrane protein activity in biosensor arrays. 978-1-4244-4122-8/11/$26.00 ©2011 IEEE 4796 33rd Annual International Conference of the IEEE EMBS
doi:10.1109/iembs.2011.6091188 pmid:22255411 dblp:conf/embc/MuRLM11 fatcat:kpyqehjxmfdelffv5rbpqztp5q