Background: Cervical cancer is one of the principal causes of cancer death among women worldwide. It is the second most common cancer and the leading cause of cancer-related death in Ethiopian women; about 77.6% of women died of 6,294 new cases reported in 2019. Early screening for cervical cancer has substantial advantage to reduce the incidence, morbidity, and mortality attributed to cervical cancer. So far, there are limited evidence on the level of cervical cancer screening uptake and its

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... terminant in low- and middle-income countries including Ethiopia. Consequently, the current study aimed to assess the level of cervical cancer screening uptake and its determinant among women of Ambo town, western Oromia, Ethiopia.Methods: A community-based cross-sectional study was conducted among 422 women aged 20-65years. Systematic random sampling was employed to recruit the eligible women from randomly selected sub-districts found in Ambo town. Interviewer-administered questionnaire was used to collect the data. Epi info was used for data entry and management from which it was exported to SPSS version 25 for detailed analysis. Variables in binary logistic regression with a p-value <0.25 were candidate for multivariable logistic regression. Estimates were presented using odds ratios (ORs) with 95% CI. Statistical Significance was declared at p value<0.05.Results: In the present study 392 women were participated giving a response rate of 93%. Overall, 63% of the respondents were known the availability of cervical cancer screening service. Only 8.7% (34) of the study participants were received cervical cancer screening in their lifetime. Being in the age group of 30-39 years (AOR=3.2; 95% CI [1.22, 8.36]) and 40-49years (AOR=4.8; 95% CI [1.42, 16.41]), having cervical cancer related discussion with health care provider (AOR=3.5; 95% CI [1.17, 10.7]), and knowing availability cervical cancer screening service (AOR=2.8; 95% CI [1.03, 7.87]) were significantly associated with uptake of cervical cancer screening. Conclusion: In this study, cervical cancer screening uptake is very low. The study also highlighted important factors that affect uptake of cervical cancer screening service. Therefore, this study result call urgent interventions by all stockholders to increase cervical cancer service uptake through continues promotions and by securing its availability and accessibility for all eligible women at all levels.