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The MCBIOS 2004 conference brought together regional researchers and students in biology, computer science and bioinformatics on October 7th-9th 2004 to present their latest work. This editorial describes the conference itself and introduces the twelve peer-reviewed manuscripts accepted for publication in the Proceedings of the MCBIOS 2004 Conference. These manuscripts included new methods for analysis of high-throughput gene expression experiments, EST clustering, analysis of mass spectrometry data and genomic analysisdoi:10.1186/1471-2105-6-s2-s1 pmid:16026594 pmcid:PMC1637027 fatcat:mlohvyw4hzgflnjfdhmfmfpede