An effortless, hasty, unambiguous, robust, exact as well as precise isocratic high performance liquid chromatographic technique has been urbanized in addition to validated for the assessment of simultaneous estimation of Metformin Hydrochloride and Metoprolol Tartrate from the plasma samples. The chromatographic severance was accomplished on Phenomenex kinetex C18 (250mm×4.6mm i.d, 5µm) column by means of a mobile phase mixture containing methanol: buffer of pH 6.8: ACN in the proportion of

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... 3:30 respectively at a flow rate of 1ml/min with injection volume of 20µl and recognition wavelength of 265 nm at ambient temperature. Retention time of Metformin Hydrochloride and Metoprolol Tartrate were found to be 2.312min and 3.055min. %RSD of the Metformin Hydrochloride and Metoprolol Tartrate were and found to be 0.7 and 0.3 respectively. %Recovery was obtained as 99.99% and 100.29% for Metformin Hydrochloride and Metoprolol Tartrate respectively. LOD, LOQ values obtained from regression equations of Metformin Hydrochloride and Metoprolol Tartrate were 1.05, 3.19 and 0.53, 1.61 respectively. Regression equation of Metformin Hydrochloride is y = 43247x + 27581.and Metoprolol Tartrate is y = 27862x + 5499.7. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries. Keywords: Metformin Hydrochloride, Metoprolol Tartrate Assay, HPLC, Plasma