Highly Specific Electrochemical Analysis of Cancer Cells using Multi-Nanoparticle Labeling

Ying Wan, Yi-Ge Zhou, Mahla Poudineh, Tina Saberi Safaei, Reza M. Mohamadi, Edward H. Sargent, Shana O. Kelley
2014 Angewandte Chemie International Edition  
Circulating tumor cells (CTCs) can be collected noninvasively and provide a wealth of information about tumor phenotype. For this reason, their specific and sensitive detection is of intense interest. Herein, we report a new, chipbased strategy for the automated analysis of cancer cells. The nanoparticle-based, multi-marker approach exploits the direct electrochemical oxidation of metal nanoparticles (MNPs) to report on the presence of specific surface markers. The electrochemical assay allows
more » ... imultaneous detection of multiple different biomarkers on the surfaces of cancer cells, enabling discrimination between cancer cells and normal blood cells. Through multiplexing, it further enables differentiation among distinct cancer cell types. We showcase the technology by demonstrating the detection of cancer cells spiked into blood samples.
doi:10.1002/anie.201407982 pmid:25283158 fatcat:tcenmf52bbdf5k3megzds6pzue