Imaging Monitoring Method of CaMKII Activity by Immunohistochemical Analysis in Schizophrenic Model Rats

Yasushi Yabuki, Osamu Nakagawasai, Takeshi Tadano, Kohji Fukunaga
2013 Yakugaku zasshi  
The authors declare no con‰ict of interest. a 東北大学大学院薬学研究科薬理学分野(〒980 8578 仙台市青葉区荒巻字青葉 6 3) , b 東北薬科大学薬学部 薬理学教室(〒981 8558 仙台市青葉区小松島 4 4 1) , c 金沢大学医薬保健学総合研究科環境健康科学(〒920 0942 金沢市小立野 5 11 80)  e-mail: kfukunaga@m.tohoku.ac.jp 本総説は,日本薬学会第 132 年会シンポジウム S28 で 発表したものを中心に記述したものである. 501 YAKUGAKU ZASSHI 133(5) 501-506 (2013)  2013 The Pharmaceutical Society of Japan -Review- Schizophrenia is characterized by various behavioral abnormalities including cognitive dysfunction. Neonatal ventral
more » ... onatal ventral hippocampus (NVH)-lesioned rats had been known as neurodevelopmental animal model similar to schizophrenia. Previous observations indicate that postpubertal NVH-lesioned rats exhibit impairments in prepulse inhibition (PPI), spontaneous locomotion, social interaction behavior and working memory. Here, we document the neurochemical basis of those defects in NVH-lesioned rats. Since Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), which is NMDA receptor downstream kinase, is essential for memory and learning acquisition, we developed a protocol to monitor the spatial changes in CaMKII autophosphorylation using immunohistochemical imaging of whole brain slices with anti-autophosphorylated CaMKII antibody in order to address mechanisms underlying impaired cognitive function in NVHlesioned rats. Immunohistochemical analyses using anti-autophosphorylated CaMKII antibody revealed that CaMKII autophosphorylation was signiˆcantly reduced in the medial prefrontal cortex (mPFC) of NVH-lesioned rats compared with control animals. This immunohistochemical technique is useful to investigate temporal and special changes in CaMKII activity in rodent brain and to evaluate drugs to improve the cognitive impairment.
doi:10.1248/yakushi.12-00278-3 pmid:23649390 fatcat:n4nyo3jcvnhhdns36fwfsikgie