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Site-specific Mutagenesis and Domain Substitutions in the Loading Module of the Nystatin Polyketide Synthase, and Their Effects on Nystatin Biosynthesis inStreptomyces noursei
Journal of Biological Chemistry
The loading module for the nystatin polyketide synthase (PKS) in Streptomyces noursei is represented by the NysA protein composed of a ketosynthase (KS S ), acyltransferase, dehydratase, and an acyl carrier protein. The absolute requirement of this protein for initiation of nystatin biosynthesis was demonstrated by the in-frame deletion of the nysA gene in S. noursei. The role of the NysA KS S domain, however, remained unclear, since no data on the significance of the "active site" serinedoi:10.1074/jbc.m212611200 pmid:12594224 fatcat:ddv3vcev45brvjv3lmackzvr5u