HPLC ANALYSIS OF PHENOLIC ACIDS AND ANTIOXIDANT ACTIVITY OF SOME CLASSICAL AYURVEDIC GUGGULU FORMULATIONS
International Journal of Research in Ayurveda and Pharmacy
Classical guggulu formulations have been used as mainstay therapeutics in chronic inflammatory diseases in Ayurvedic system of medicine since centuries. The present study included six guggulu formulations such as Kaishora guggulu, Kanchanara guggulu, Yogaraj guggulu, Punrnava guggulu, Amritadi guggulu and Trayodasanga guggulu.Phenolic compounds play a vital role as evident from phenolic and flavonoid content in guggulu drugs as well as antioxidant activity assayed by DPPH radical scavenging
... ical scavenging activity and FRAP antioxidant power. Yograj guggulu was found to have strong reducing ability (1156.76 µM Fe(II)/mg) among six drugs chosen in this investigation and Amritadi guggulu has revealed powerful scavenger of DPPH radicals (IC50:1.05 mg/ml). Phenolic acid composition for each guggulu drug was analyzed and twelve phenolic acid compounds were identified and estimated by HPLC. Gallic acid (8.29-49.28 mg/g) was found in high concentration and ellagic acid (0.69-6.02 mg/g) was found to the next in all formulations. Other phenolic acid components estimated were protocatechuic acid, p-hydroxybenzoic acid, gentisic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, sinapic acid in all guggulu formulations. A synergistic activity towards therapeutic efficacy was proposed on the basis of phenolic acid composition in each guggulu formulation.