Determinação de lamivudina, estavudina e nevirapina, em comprimidos, por cromatografia líquida de alta eficiência

Gisele Rodrigues da Silva, Gustavo Portela Lages, Gerson Antônio Pianetti, Elzíria de Aguiar Nunan, Cristina Duarte Vianna Soares, Ligia Maria Moreira de Campos
2006 Química Nova  
Recebido em 23/2/05; aceito em 24/3/06; publicado na web em 1/8/06 DETERMINATION OF LAMIVUDINE, STAVUDINE AND NEVIRAPINE IN TABLETS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. A high performance liquid chromatography method was developed to quantify lamivudine, stavudine and nevirapine combined in tablets. The separation was carried out in less than 10 min using a phosphate buffer of pH 3.0 and acetonitrile (75:25, v/v) as mobile phase, a LiChrospher ODS column and UV detection at 266 nm. The
more » ... hod was linear over the range of 15-135 μg/mL (lamivudine), 4-36 μg/mL (stavudine) and 20-180 μg/mL (nevirapine). The accuracy ranged from 98.56 to 102.04% and intra-day and inter-day precision was less than 1% for the three drugs. The method showed robustness, remaining unaffected by deliberate variations in relevant parameters.
doi:10.1590/s0100-40422006000600002 fatcat:6q3m7xps4racvavpgqnppyrs2q