TGFβ1a regulates zebrafish posterior lateral line formation via Smad5 mediated pathway

Cencan Xing, Bo Gong, Yu Xue, Yanchao Han, Yixia Wang, Anming Meng, Shunji Jia
2015 Journal of Molecular Cell Biology  
The zebrafish sensory posterior lateral line (pLL) has become an attractive model for studying collective cell migration and cell morphogenesis. Recent studies have indicated that chemokine, Wnt/b-catenin, Fgf, and Delta-Notch signaling pathways participate in regulating pLL development. However, it remains unclear whether TGFb signaling pathway is involved in pLL development. Here we report a critical role of TGFb1 in regulating morphogenesis of the pLL primordium (pLLP). The tgfb1a gene is
more » ... ndantly expressed in the lateral line primordium. Knockdown or knockout of tgfb1a leads to a reduction of neuromast number, an increase of inter-neuromast distance, and a reduced number of hair cells. The aberrant morphogenesis in embryos depleted of tgfb1a correlates with the reduced expression of atoh1a, deltaA, and n-cadherin/cdh2, which are known important regulators of the pLLP morphogenesis. Like tgfb1a depletion, knockdown of smad5 that expresses in the pLLP, affects pLLP development whereas overexpression of a constitutive active Smad5 isoform rescues the defects in embryos depleted of tgfb1a, indicating that Smad5 mediates tgfb1a function in pLLP development. Therefore, TGFb/Smad5 signaling plays an important role in the zebrafish lateral line formation.
doi:10.1093/jmcb/mjv004 pmid:25603803 fatcat:mumgd37luffhzm53u25etcw2pe