Comparative Study of Operation Performance and Microbial Community between Chem-bioflocculation(CBF) Process and Chemically Enhanced Primary Treatment (CEPT) Process

Siqing Xia, Feng Wang, Yan Shi, Yingfu Rao, Dianhai Yang
2006 Proceedings of the Water Environment Federation  
Chem-bioflocculation (CBF) process was a new municipal wastewater treatment process developed in recent years. The flocculant dosage was one of the most important factors in operation optimization of chem-bioflocculation(CBF) treatment process. Five levels of flocculant dosage (PAFC) from 30mg/L to 100mg/L were applied to compare operational performance between CBF process and chemically enhanced primary treatment (CEPT) process. Superior column loadings of COD, SCOD, SS, and NH4+-N were
more » ... NH4+-N were obtained in CBF process. The column loading of TP was present as close level in two processes. The biodiversities of microbial communities, including Bacteria, Acitnomycetes, Acidobacterium, and ammonia oxidizing bacteria (AOB), were analyzed by using PCR-DGGE method with group-specific primers at different flocculant dosage of polyaluminium ferric chloride (PAFC). The results indicated that CBF reactor contained special bacterial community structure other than CEPT reactor. The special distribution of bacterial population was even at different positions of two reactors. To quantify Bacteria cells, Real-Time PCR method was applied by using primers for 16SrDNA V3 regions. CBF reactor had a little less population size of Bacteria than traditional AS process, but great higher than that of CEPT reactor. Figure 2 Column loadings for primary contaminations in CBF and CEPT reactors The DGGE patterns for Bacteria, Acitnomycetes, Acidobacterium and AOB in two reactors were described in Figure 3 . As shown, two processes both had special community structure. Some obvious bands were uniquely found in CEPT reactor, such as Band Act 1-3 (Figure 3b ), Acd 1-2 (Figure 3c ), and AOB 1-8 (Figure 3d ), indicating that CBF process had selectivity for some specific bacterial species. For the two reactors was fed with the same influent, so they both had identical continued inoculation from influent. The bacterial compositions in CEPT process might be result from selection of flocculent dosage (70mg/L Polymeric AlCl 3 , FeCl 3 , PAFC) because no obvious favorable condition, such as aeration, was designed in 2010 WEFTEC®.06
doi:10.2175/193864706783750574 fatcat:rjkfnxu75vfh5grn6guuxwqa6a