Objective: This study aimed to investigate the possible protective effect of olive leaf extract on liver and blood parameters in methotrexate (MTX)-induced toxicity in rats. Methods: Normal control rats were injected I.P with (1 ml of distilled water) once daily. Group II: Rats were injected orally by gavage with olive leaves extract (of 0.5 g/kg body wt) once daily. Group III: Rats were injected intraperitoneally with MTX of 20mg/kg once in the day 12th day. Group IV: Rats were injected orally

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... by gavage with olive leave extract (0.5 g/kg body weight) once daily followed by I.P. injection of MTX a single dose of 20 mg/kg in the 12th day.On the 14 th day, rats were sacrificed by decapitation. Whole blood collected and liver tissues were kept in formalin and glutaraldehyde fixatives for microscopic and ultrastructural examination respectively. Results: This study showed that hepatocellular damage was clearly more evident in the MTX group than the MTX+Olive leaf extract group besides, MTX administration elevated AST , ALT and LDH activities whereas they were depressed with olive leaf extraction administration and this was proofed ultra-structurally. Conclusion: The present study indicates that olive leaf extract may be thetherapeutic benefit agentagainst MTX-cytotoxicity. Abd El-Azim AO. Antioxidant effect of olive leaf extract on methotrexate-induced hepatic injury in rats. Canad J Clin Nutr 2014; 2 (1): 4-14