Diarrhea is a major contributor to childhood mortality and morbidity in the developing world. Objective: The study was aimed to determine the prevalence of Entamoeba histolytica among children diagnosed with acute diarrhea in Kura General Hospital Kano, from February to August 2017. Methodology: A total of 236 stools samples were collected from children within the age group of 0 – 5 years diagnosed with acute diarrhea attending the Hospital. The stool samples were examined for Entamoeba

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... ica cysts and trophozoites using direct wet preparation and Formol Ether concentration techniques. Results: The result showed that 23 samples which accounted for 9.75% of the samples were found to be positive for the parasite. Highest incidence of the parasite was found among children within the age category of 4 - 5 years. The results also indicated that males (56.5%) were more infected than females (43.5%). However, there is no statistical difference in the rate of infection among the sex and age group of the patients at p< 0.05. Conclusion: In conclusion, maintenance of personal hygiene and improved sanitation of the environment will indeed prevent contamination of food and water sources.