In the present study, the antioxidant activity of successive solvent extracts of evaluated by different in vitro antioxidant assay models. The prepared solvent extracts were screened for the antioxidant and radical scavenging activity on ABTS radical cation, DPPH free radical, hydrogen peroxide, superoxide radical and hydroxyl radical. Among the prepared extracts, ethanol and ethyl acetate extracts showed prominent activity against the tested models. Aqueous extract show ethyl acetate. Hexane

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... tracts fails to exhibit the antioxidant and radical scavenging activity in all the tested models. Chloroform extract did not produce significant activity. The antioxidant and free radic activities of the extracts were compared with standard antioxidants like ascorbic acid and rutin. Preliminary phytochemical screening revealed that ethanol and ethyl acetate extracts are rich in flavonoids, polyphenols, tannins and saponins. The radical scavenging and antioxidant activity of the extracts may be due to the presence of these kinds of phytochemicals in the extract. Therefore, the plant could be considered as a very good antioxidant source with therapeutic potential. Oxidation and reduction reactions are essential to many living organisms for the production of energy to biological purposes. However, oxygen free ra species (ROS) which are continuously produced in vivo, result in cell death and tissue damage. These species can react with biological substrates such as DNA and proteins, leading to several diseases including cancer, diabe and atherogenesis 1,2. Antioxidants are vital substances which provide protection to living organisms from damage caused by uncontrolled production of ROS and the concomitant lipid peroxidation, protein damage antinecrotic, neuroprotective, chemopreventive and hepatoprotective drugs have recently been shown to have antioxidant and radical scavenging mechanism as part of their activity There is an increased interest in natural antioxidants present in medicinal and dietary plants, which might help to prevent oxidative damage WWW.IJAPBR.COM In the present study, the antioxidant activity of successive solvent extracts of Indigofera astragalina antioxidant assay models. The prepared solvent extracts were screened for the enging activity on ABTS radical cation, DPPH free radical, hydrogen peroxide, superoxide radical and hydroxyl radical. Among the prepared extracts, ethanol and ethyl acetate extracts showed prominent activity against the tested models. Aqueous extract showed moderate activity in all the models next to ethyl acetate. Hexane extracts fails to exhibit the antioxidant and radical scavenging activity in all the tested models. Chloroform extract did not produce significant activity. The antioxidant and free radic activities of the extracts were compared with standard antioxidants like ascorbic acid and rutin. Preliminary phytochemical screening revealed that ethanol and ethyl acetate extracts are rich in flavonoids, polyphenols, The radical scavenging and antioxidant activity of the extracts may be due to the presence of these kinds of phytochemicals in the extract. Therefore, the plant could be considered as a very good antioxidant source with therapeutic potential.