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Objective: We describe a new method of immortalizing amniocytes with chromosome abnormalities to generate renewable resources for studying rare diseases. Methods: Three methods were investigated by randomly dividing 180 cases of adherent amniocytes into groups A, B, and C. Group A cells were digested with 0.25% trypsin for 10, 20, 30, 60, 90, and 120 mins. Group B and C cells were digested with 0.25% trypsin for 3-5 minutes. Group A and B cells were then transfected with PT67 cell-produceddoi:10.21203/rs.2.17757/v1 fatcat:677phbkeknhojomfq4fqaxsbbe