Research of Quality Standards for Stachydrine Hydrochloride in Chinese Medicine TJF Granule

Yao Lu, Wei Yu, Pingniang Shen, T. J. Khoo, C. Wiart
2013 Pharmacology and Pharmacy  
A high-performance liquid chromatographic method was applied to the determination of stachydrine hydrochloride concentration in TJF granule (Chinese name: Tiao-Jing-Fang), using a mobile phase of methanol-acetonitrile (50:50, v/v) by the Agilent Kro-masi NH 2 column (250 mm × 4.6 mm, 5 μm, S/N: 22N25110). Detection wavelength was 201 nm. The result revealed good linearity of stachydrine hydrochloride and was obtained within the range of 0.20 -1.98 μg/mL (R = 0.9995). The average recovery was
more » ... 01%; the relative standard deviation (RSD) was 0.19%. To the best of our knowledge, this is the first report dedicated to the determination of stachydrine hydrochloride by the evaporative light scattering detector-high-performance liquid chromatographic (ELSD-HPLC) method.
doi:10.4236/pp.2013.43040 fatcat:3qhuqcckw5ajfkhqe5ci7xsb34