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Kinetic Parameters for the Vesicular Acetylcholine Transporter: Two Protons Are Exchanged for One Acetylcholine†
1998
Biochemistry
The vesicular acetylcholine transporter (VAChT) mediates ACh storage in synaptic vesicles by exchanging cytoplasmic ACh with vesicular protons. This study sought to determine the stoichiometry of exchange by analysis of ligand binding and transport kinetics. The effects of different pH values inside and outside, external ACh concentrations, and electrical potential gradients on ACh transport by vesicles isolated from the electric organ of Torpedo were determined using a pH-jump protocol. The
doi:10.1021/bi9802263
pmid:9748347
fatcat:sjgzlyvejjcnzbnoyrtzymjfpm