Effects of bovine serum albumin on the ability of Barbour-Stoenner-Kelly medium to detect Borrelia burgdorferi

S M Callister, K L Case, W A Agger, R F Schell, R C Johnson, J L Ellingson
1990 Journal of Clinical Microbiology  
The ability of decreasing inocula of Borrelia burgdorferi to grow in otherwise identical Barbour-Stoenner-Kelly (BSK) media containing different lots of bovine serum albumin (fraction V) was determined. These media differed significantly in ability to detect B. burgdorferi. Some BSK media required inocula of 2 x I15 organisms per ml for detection, while other media could stimulate growth after inoculation with <2 organisms per ml. In addition, organisms from the less sensitive BSK media were
more » ... nner, longer, and less tightly coiled. The endpoint dilutions of indirect fluorescent-antibody titers, especially immunoglobulin M, exhibited up to 16-fold decreases, and both immunoglobulin G and M titers were more difficult to interpret with diagnostic slides prepared from some longer, thinner B. burgdorferi. These results demonstrate that, when performing laboratory investigations which rely on B. burgdorferi, it is essential that the quality of the BSK medium be determined.
doi:10.1128/jcm.28.2.363-365.1990 fatcat:coraz6snl5b6lnd7hrxac6qh6y