Effect of prostaglandin A1 in the induction of stress proteins in Aedes albopictus cells
Brazilian Journal of Medical and Biological Research
Prostaglandins are natural fatty acid derivatives with diverse physiological effects, including immune function and the control of cell growth. While the action of prostaglandins in the induction of stress proteins in vertebrate cells is well documented, their functions in invertebrate cells have been poorly investigated. The purpose of the present study was to investigate the effect of prostaglandin A 1 (PGA 1 ; 0.25, 1.25 and 12.5 µg/ml) on protein synthesis during the growth of Aedes
... th of Aedes albopictus cells. We found that PGA 1 stimulates the synthesis of several polypeptides with molecular masses of 87, 80, 70, 57, 29, 27 and 23 kDa in Aedes albopictus cells. When the proteins induced by PGA 1 and those induced by heat treatment were compared by polyacrylamide gel electrophoresis, PGA 1 was found to induce the stress proteins. The HSP70 family and the low-molecular weight polypeptides (29 and 27 kDa, respectively) were induced by PGA 1 in the lag phase. We also observed that PGA 1 is able to induce a 23-kDa polypeptide independently of the growth phase of the cell.