DNA CONTENT OF MYCOBACTERIA AND NOCARDIAS
MycobacteriumおよびNocardiaのDNA含有量の比較

Michio TSUKAMURA, Shoji MIZUNO
1969 Kekkaku (Tuberculosis)  
The content of deoxyribunucleic acids (DNA) was compared in various mycobacteria by Tsukamuras), but, since the data were presented inadequately, it was desired again to estimate the DNA content, applying a more appropriate method, in various mycobacteria and nocardias. Twelve strains of 7 species of Mycobacterium and 12 strains of 5 species of Nocardia were used (Table 1 and 2 ). The strains had been maintained on Ogawa egg medium at -20°C. After subcultureing test strains on Ogawa egg medium
more » ... t 37'C (Mycobacterium)or at 28°C(Nocardia) , growing organisms were harvested, washed three times in distilled water, and then used for estimation of DNA, ribonucleic acid (RNA) and protein contents. Rapid-growing mycobacteria (Table 3 ) and nocardias were harvested after incubation for 7 days, slow-growing mycobacteria (Table 3 ) after incubation for 14 days, and very slowly growing mycobacteria (Table 3 ) after incubation for 21 days.
doi:10.11400/kekkaku1923.44.6_155 fatcat:qpudzypjmjgqvghhbc3x5pp64a