PCR Using 3′-Mismatched Primers To Detect A2142C Mutation in 23S rRNA Conferring Resistance to Clarithromycin in Helicobacter pylori Clinical Isolates

Teresa Alarcón, Diego Domingo, Nuria Prieto, Manuel López-Brea
2000 Journal of Clinical Microbiology  
Twenty-five clarithromycin-resistant Helicobacter pylori strains (selected by agar dilution) were studied to detect A2142G and A2143G mutations in the 23S rRNA gene by a PCR-restriction fragment length polymorphism method and an A2142C mutation by PCR using a 3′-mismatched specific primer. A 700-bp amplified fragment was obtained by the mismatched PCR only in strains without an A2142G or A2143G mutation, indicating that those strains had the A2142C mutation.
doi:10.1128/jcm.38.2.923-925.2000 fatcat:xdr2bs25j5h4nhbjb7yafpujjq