Aspirin, one of the oldest and most widely used anti-inflammatory drugs, has lately been found to lessen the incidence of cancer. The main pharmacological effects of aspirin are thought to be generated by its covalent alteration of cyclooxygenase-2 (COX-2) via acetylation of Ser530. However, the exact biochemical mechanism and specificity of how it works have yet to be figured out. In modern times, there has been a lot of research done on the qualities of spectra for pharmaceuticals (such as

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... orescence), and the goal of this research is to display more physicochemical features of molecules. Anthracene is colorless, but when exposed to ultraviolet (UV) light, it fluoresces blue (with a maximum emission range of 400–450 nm). The ratio of the number of photons that are emitted to the number of photons that are absorbed can be used as a measurement of the quantum yield, which is a measurement of the efficiency of photon emission. The quantum yield ratio is the name given to this particular ratio. In the present work, we studied absorption spectra by spectrophotometer and fluorescence by spectrofluorometer photometer from UV to visible range for aspirin. We found that aspirin fluoresces (max = 370–470 nm) under UV light.