We wanted to take stock of the new microbio-epidemiological trend of mixed urolithiasis infected or colonized by multi-resistant germs including multi-resistant enterobacteria bacteria extended spectrum β-lactamase and others. Materials and Methods: Assess the impact of the microbiological study of the microbiological study (phenotypic and molecular) of pyelic urine and/or stone compared to standard urineculture (ECBU). Results: 114 (03%) had an infected (or colonized) stone. Preoperative ECBU

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... as 90% negative compared at the culture of urine pyelique and stone with 22% Proteus mirabilis, 15% Staphylococcus aureus, 11% K. pneumoniae and others emerging species. Antibiotic resistance detected in the stone and pyelic urine analysis: 60% of E. coli and 40% of K. pneumoniae resistant to Cefazolin (CIIG) with 60% resistance to ciprofloxacin in E. coli and 20% in K. pneumoniae. we had five extended spectrum β-lactamase strain: tow Enterobacter cloacae complex (ECC) and three K. pneumoniae. Conclusion: Mixed infectious urolithiasis can be a real public health problem because, pathogenic germs sometimes alarmist due to their resistance to antibiotics and their etiopathogens or virulences and can be life-threatening for patients with additional costs, especially hospital and social care.