Pyochelin biotransformation shapes bacterial competition [article]

C. Jenul, K. Keim, J. Jens, M. J. Zeiler, K. Schilcher, M. Schurr, C. Melander, V.V. Phelan, A.R. Horswill
2022 bioRxiv   pre-print
AbstractIt has become evident that numerous infections are polymicrobial and Pseudomonas aeruginosa and Staphylococcus aureus are among the most frequently isolated co-infecting bacterial species. We applied mass spectrometry guided interaction studies to determine how chemical interaction shapes the fitness and community structure during co-infection of these two pathogens. We demonstrate that S. aureus is equipped with an elegant mechanism, inactivation of pyochelin via the yet
more » ... methyltransferase Spm (staphylococcal pyochelin methyltransferase), to increase its survival during in vivo competition with P. aeruginosa. Methylation of pyochelin abolishes the siderophore activity of pyochelin and significantly lowers pyochelin-mediated intracellular ROS production in S. aureus. In a murine wound co-infection model, a S. aureus mutant unable to metyhlate pyochelin shows significant lower fitness compared to its parental strain.One-Sentence SummaryStaphylococcus aureus inactivates pyochelin to increase its survival during in vivo competition with Pseudomonas aeruginosa.
doi:10.1101/2022.04.18.486787 fatcat:hzp4ecpopjbg7dwelypne5oetq