Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems

Md Abdul Kader Sagar, Bing Dai, Jenu V. Chacko, Joshua J. Weber, Andreas Velten, Scott T. Sanders, John G. White, Kevin W. Eliceiri
2019 Journal of Biomedical Optics  
The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection. We present a fiber-based method to obtain spectral information from a multiphoton fluorescence lifetime imaging (FLIM)
more » ... . This is made possible using the time delay introduced in the fluorescence emission path by a dispersive optical fiber coupled to a detector operating in time-correlated single-photon counting mode. This add-on spectral implementation requires only a few simple modifications to any existing FLIM system and is considerably more cost-efficient compared to currently available spectral detectors.
doi:10.1117/1.jbo.25.1.014506 pmid:31833280 pmcid:PMC6907392 fatcat:gssxq3eup5gk5jykhel7yo7sgu