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The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection. We present a fiber-based method to obtain spectral information from a multiphoton fluorescence lifetime imaging (FLIM)doi:10.1117/1.jbo.25.1.014506 pmid:31833280 pmcid:PMC6907392 fatcat:gssxq3eup5gk5jykhel7yo7sgu