Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp

YOPI YOPI, APRIDAH CAMELIAWATI DJOHAN, NANIK RAHMANI, ALIFAH MAFATIKHUL JANNAH
2017 Biofarmasi Journal of Natural Product Biochemistry  
Yopi, Djohan AC, Rahmani N, Jannah AM. 2017. Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp. Biofarmasi (Rumphius J Nat Prod Biochem) 15: 15-20. Isolation, identification, and characterization of mannanase, xylanase and cellulase producing indigenous marine bacteria have been conducted from total 20 isolates. Based on 16S rDNA sequence analysis, three potential isolates are obtained and identified as Bacillus subtilis (M8), Bacillus
more » ... ), Bacillus tequilensis (X4) and Bacillus cereus (C9). The potential strains M8, X4 and C9 can produce mannanase, xylanase and cellulase activities such as 9.5 U/mL; 0.36U/mL;0.56U/mL with optimum pH and temperature 6.0;50oC, 5.5;70oC and 8;50oC, respectively. Based on the TLC analysis, mannanase from M8 and xylanase from X4 has potential to hydrolyzed mannan and xylan for producing oligosaccharides with size around tri-hexasaccharides as a main product.
doi:10.13057/biofar/f150103 fatcat:qz77yfidkjeejepwv2afc4cqoq